Biochar-derived persistent free radicals and reactive oxygen species reduce the potential of biochar to mitigate soil N2O emissions by inhibiting nosZ----中国科学院遗传发育所农业资源研究中心

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论文题目	Biochar-derived persistent free radicals and reactive oxygen species reduce the potential of biochar to mitigate soil N2O emissions by inhibiting nosZ
作　　者	吴萍，谢明慧， Clough, Tim J，袁丹，吴思敏，何小冬，胡春胜，周顺桂，秦树平;
发表年度	2023
刊物名称	SOIL BIOLOGY & BIOCHEMISTRY
卷、期、页码	178; ;
影响因子
论文摘要	Biochar amendment has been proven to generally reduce soil nitrous oxide (N2O) emissions. However, the effects and underlying mechanisms of biochar-derived reactive oxygen species (ROS) on soil N2O emissions are still unclear. Thus, we firstly weakened the intensities of persistent free radicals (PFRs) within biochar using trie-thanolamine (TEA) as a quencher, and then used soil incubation methods to compare the potentials of TEA -quenched and un-quenched biochar in mitigating soil N2O emissions. The TEA-quenched biochar generated less hydrogen peroxide (H2O2) and hydroxyl radical (center dot OH), while having a higher soil N2O emission mitigation potential, than the un-quenched biochar. The N2O emissions and the N2O/(N2O + N2) ratio were significantly, positively, correlated with the generated H2O2 and center dot OH contents. These results demonstrated that biochar-derived ROS weakened biochar's mitigation of soil N2O emissions. The specific mechanisms of biochar-derived ROS on soil N2O emissions were further explored by a ROS scavenging experiment. It was found that scavenging H2O2 by catalase efficiently hindered the generation of center dot OH, resulting in decreases in N2O emissions and the N2O/(N2O + N2) ratio. Meanwhile, biochar-derived ROS exhibited a more severe inhibition on N2O reductase gene (nosZ) expression than that on the expression of genes responsible for N2O production (nirK and nirS), indicating that biochar-derived ROS weakened biochar's mitigation of soil N2O emissions by inhibiting microbial N2O reduction. Our results imply that controlling the content of biochar-derived ROS is a promising strategy to maximize biochar's potential for mitigating soil N2O emission.
全文链接	http://dx.doi.org/10.1016/j.soilbio.2023.108970
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