Identification and Dynamic Regulation of microRNAs Involved in Salt Stress Responses in Functional Soybean Nodules by High-Throughput Sequencing----中国科学院遗传发育所农业资源研究中心

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论文

论文题目	Identification and Dynamic Regulation of microRNAs Involved in Salt Stress Responses in Functional Soybean Nodules by High-Throughput Sequencing
作　　者	Dong, ZH (董章辉); Shi, L (石磊); Wang, YW (Wang, Yanwei); Chen, L (Chen, Liang); Cai, ZM (Cai, Zhaoming); Wang, YN (王幼宁); Jin, JB (Jin, Jingbo); Li, X (李霞);
发表年度	2013
刊物名称	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷、期、页码	卷: 14; 期: 2; 页: 2717-2738
影响因子
论文摘要	Both symbiosis between legumes and rhizobia and nitrogen fixation in functional nodules are dramatically affected by salt stress. Better understanding of the molecular mechanisms that regulate the salt tolerance of functional nodules is essential for genetic improvement of nitrogen fixation efficiency. microRNAs (miRNAs) have been implicated in stress responses in many plants and in symbiotic nitrogen fixation (SNF) in soybean. However, the dynamic regulation of miRNAs in functioning nodules during salt stress response remains unknown. We performed deep sequencing of miRNAs to understand the miRNA expression profile in normal or salt stressed-soybean mature nodules. We identified 110 known miRNAs belonging to 61 miRNA families and 128 novel miRNAs belonging to 64 miRNA families. Among them, 104 miRNAs were dramatically differentially expressed (>2-fold or detected only in one library) during salt stress. qRT-PCR analysis of eight miRNAs confirmed that these miRNAs were dynamically regulated in response to salt stress in functional soybean nodules. These data significantly increase the number of miRNAs known to be expressed in soybean nodules, and revealed for the first time a dynamic regulation of miRNAs during salt stress in functional nodules. The findings suggest great potential for miRNAs in functional soybean nodules during salt stress.
全文链接	http://dx.doi.org/10.3390/ijms14022717
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